Journal: Cancer Research
Article Title: Regression of Melanoma in a Murine Model by RLIP76 Depletion
doi: 10.1158/0008-5472.can-05-3534
Figure Lengend Snippet: Figure 1. Comparison of RLIP76 levels in cultured malignant cells versus nonmalignant cells. Aliquots of crude detergent extracts of the membrane fractions of malignant cells (H1618, H358, OVCAR-3, PC-3, MCF-7, B16, HepG2, and DG-1) and nonmalignant cells (HAVSM, HUVEC, HLMVEC, and HLBEC), containing 200 Ag protein were used for SDS-PAGE and Western blotting against anti-RLIP76 IgG as primary antibody and horseradish peroxidase–conjugated goat anti-rabbit IgG as secondary antibody and developed with 4-chloro-1-napthol as chromogenic substrate. Results were quantified by scanning densitometry of the full-length RLIP76 protein band near 109 kDa. h-Actin was used as an internal control.
Article Snippet: Human small cell lung cancer (SCLC) lines H1618 and non–small cell lung cancer (NSCLC) lines H358 (bronchioalveolar), PC-3 (human prostate), and B16 mouse skin melanoma were purchased from American Type Culture Collection (Manassas, VA).
Techniques: Comparison, Cell Culture, Membrane, SDS Page, Western Blot, Control